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What is the main reason to pick a single colony?

Answer and explanation

Correct answer: To obtain a pure clone culture

The direct answer is option A: to obtain a pure clone culture. A visible colony on a solid agar plate usually develops from one original cell or from a small group of genetically identical cells. By carefully picking one well-isolated colony and growing it in fresh medium, the researcher obtains a culture expected to come from one clone. This makes later plasmid preparation, restriction analysis, sequencing, or protein-expression testing more reliable. Option A is correct because a single colony reduces the chance of mixing different transformants. Option B is wrong because picking a colony is not intended to change agar color. Option C is wrong because it has nothing to do with counting water. Option D is wrong because the purpose is to grow the selected cells, not kill the host. The colony should be well isolated; picking from merged colonies can produce a mixed culture and confuse results. Also, “pure” means genetically uniform as intended, not that every cell is proven identical without testing. Memory cue: one isolated colony, one likely clone, clearer results.

Related tags

Single ColonyPure CloneClone Culture

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