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What effect can low transformation efficiency have on a library?

Answer and explanation

Correct answer: Some desired clones may be missed

The governing concept is representation of DNA diversity in a recombinant library. Transformation efficiency is the fraction of cells that successfully receive and maintain recombinant DNA. If efficiency is low, the number of independent transformants is smaller, so some DNA fragments or rare sequences from the original pool may never enter a cell or may not be recovered during colony sampling. The resulting library can therefore be incomplete and less representative. Option A is correct. A perfect library is not guaranteed, and DNA does not become water; the process also clearly affects library coverage, so B, C, and D are incorrect.

Related tags

Transformation EfficiencyGenomic LibraryClone RepresentationCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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