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Lower expression temperature can help in what?

Answer and explanation

Correct answer: Protein folding and solubility

The governing concept is optimization of heterologous protein expression. Lowering the induction or expression temperature often slows translation and reduces the rate at which newly made polypeptides accumulate. The extra time can allow some proteins to fold correctly with the help of cellular chaperones, increasing soluble protein and decreasing aggregation into insoluble inclusion bodies. This is not universal, because the best temperature depends on the host, vector, and protein, but it is a common optimization strategy. Option A is correct; low temperature does not inherently destroy DNA, burn the host, or remove a genetic marker.

Related tags

Expression TemperatureProtein FoldingSolubilityCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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