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What is the simple meaning of an auxotrophic marker?

Answer and explanation

Correct answer: Using a nutrient requirement in selection

The governing concept is nutritional selection based on auxotrophy. An auxotrophic host has a mutation that prevents it from synthesizing a particular essential compound, so it cannot grow on a medium lacking that nutrient. If a vector carries a functional gene that complements the defect, transformed cells regain the ability to grow under the selective condition. Consequently, the nutrient requirement becomes a convenient marker for identifying transformants. Option A is correct. DNA cutting is performed by restriction enzymes, protein staining is an analytical method, and making water is unrelated to marker function; therefore B, C, and D are wrong.

Related tags

Auxotrophic MarkerNutritional SelectionYeastCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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