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What type of selectable marker can be used in yeast transformation?

Answer and explanation

Correct answer: Nutritional marker or drug-resistance marker

The governing concept is selection of transformed yeast cells by a marker that produces a detectable growth advantage. A nutritional, or auxotrophic, marker complements a metabolic defect in the host; when the cells are grown on medium lacking the required nutrient, only transformants carrying the functional marker can grow. Yeast can also be selected with suitable drug-resistance genes when the experimental strain and conditions permit. Therefore option A is correct. Chlorophyll, water, and bone are not standard selectable-marker systems for yeast transformation, making B, C, and D incorrect.

Related tags

Yeast TransformationSelectable MarkerAuxotrophyCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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