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What will happen if the host has fewer tRNAs for rare codons?

Answer and explanation

Correct answer: The ribosome may pause or translation may slow down, reducing protein expression

If the host has a low amount of tRNA matching a rare codon, the ribosome must wait for that tRNA. Translation may slow down or pause, reducing the yield of the target protein. Increased DNA replication is not a direct consequence of rare codons. Exam tip: rare-codon problems in heterologous protein expression can be reduced by codon optimization or by using a host supplemented with the required tRNAs.

Related tags

Rare CodonsTrnaTranslationProtein ExpressionCompetent HostCodon OptimizationPrinciples Of BiotechnologyBiotechnology

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