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lacZ reporter is linked with which screening?

Answer and explanation

Correct answer: Blue-white screening

The lacZ gene is associated with production of the enzyme β-galactosidase. On a medium containing X-gal, colonies with a functional lacZ gene appear blue, whereas insertion of foreign DNA can disrupt lacZ and produce white colonies. Therefore, lacZ is used in blue-white screening. Antibiotic resistance is generally associated with a selectable marker, not with the lacZ reporter. Exam tip: remember that lacZ disruption gives white colonies.

Related tags

LaczBlue-White ScreeningRecombinant DnaReporter GeneColony SelectionBiotechnologyCompetent HostPrinciples Of Biotechnology

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