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What can happen with a toxic insert in a high-copy plasmid?

Answer and explanation

Correct answer: Host growth can be affected

A high-copy plasmid is present in many copies per host cell. If its insert produces a toxic product, or if transcription and translation of the insert consume substantial cellular resources, the increased copy number can intensify the burden. The host may grow slowly, lose viability, select for deletions or mutations in the insert, or maintain fewer plasmid-bearing cells. Option B is correct because host growth can be impaired by toxicity and metabolic burden. A high-copy vector does not always improve the host, and DNA or a selectable marker cannot change into water or fat. A lower-copy vector or regulated expression may sometimes reduce the problem.

Related tags

Toxic InsertHigh-Copy PlasmidHost GrowthCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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