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Colony count is related to what?

Answer and explanation

Correct answer: Estimating transformation efficiency

After transformation, each visible colony generally develops from a viable cell or a small group of cells that multiplied on the selective medium. Counting colonies therefore provides an estimate of transformation success. Transformation efficiency is commonly expressed as the number of transformant colonies relative to the amount of DNA used, often normalized per microgram of DNA; comparison with an untreated or positive control improves interpretation. Option A is correct because colony number is evidence of viable transformants and can be used for an efficiency estimate. DNA colour, host smell, and plate thickness do not measure successful DNA uptake or plasmid maintenance.

Related tags

Colony CountTransformation EfficiencyControl PlateCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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