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What is the role of an agar plate in a transformation experiment?

Answer and explanation

Correct answer: To grow colonies and perform selection

The direct answer is D: an agar plate allows transformed cells to grow into colonies and can provide selection. After transformation, cells are spread on solid nutrient agar. Each surviving cell can divide repeatedly and form a visible colony containing many genetically similar cells. If the agar contains a suitable antibiotic, only cells carrying the resistance marker on the plasmid should grow under correct conditions, so the plate helps select transformants. Option D is correct. Option A is wrong because agar does not make DNA liquid; it solidifies the growth medium. Option B is wrong because its purpose is not to digest host cells. Option C is wrong because the plate does not give light to the plasmid; light is not the selection principle here. Selection is not the same as confirmation: a growing colony may still need screening or further testing to confirm the desired insert. Memory cue: plate, colonies, selection.

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Agar PlateColoniesSelection

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