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What happens if plasmid ori does not work in host?

Answer and explanation

Correct answer: The plasmid will not replicate and will not be stably maintained in the host

The ori (origin of replication) is the sequence from which plasmid replication begins. If the ori does not function in the host, the host replication machinery cannot replicate the plasmid DNA. Consequently, the plasmid is lost over successive cell divisions and cannot be stably maintained. It does not necessarily integrate into the host chromosome, so option A is incorrect. Exam tip: for a plasmid vector to persist in a host, its ori must be compatible with that host.

Related tags

Plasmid Origin Of ReplicationOri CompatibilityPlasmid MaintenanceCompetent HostBiotechnologyPrinciples Of BiotechnologyClass 11 Mcq

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