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What is the benefit of making aliquots of competent cells?

Answer and explanation

Correct answer: Avoiding repeated freeze-thaw

When competent cells are stored in small aliquots, only one tube is thawed when needed. The remaining cells are protected from repeated freeze-thaw cycles, which can reduce their competence and transformation efficiency. Producing antibiotics or digesting host cells is not the purpose of aliquoting. Exam tip: remember that aliquoting mainly prevents loss of cell activity due to repeated freeze-thawing.

Related tags

Competent CellsAliquotsFreeze ThawTransformation EfficiencyBiotechnology StorageCompetent HostPrinciples Of BiotechnologyBiotechnology

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