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For what is SOC medium commonly used?

Answer and explanation

Correct answer: Recovery after transformation

SOC is a nutrient-rich recovery medium commonly used after bacterial transformation. Transformation temporarily stresses cells, especially when a heat-shock or electroporation method has been used. Giving the cells time in SOC allows them to repair, express the introduced antibiotic-resistance gene, and begin recovering before they are placed on selective agar.

After this recovery period, the cells are transferred to a medium containing the appropriate antibiotic. Only cells that successfully acquired and expressed the vector marker are expected to form colonies. Therefore option D, recovery after transformation, is correct. SOC is not used primarily for cutting DNA, staining a gel, or digesting proteins; those activities require different reagents and procedures.

Related tags

Soc MediumRecoveryTransformation

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