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Why is clean plasmid DNA preferred in transformation?

Answer and explanation

Correct answer: Fewer salts, proteins, and other impurities can improve DNA uptake and competent-cell survival

In transformation, competent cells must take up the plasmid DNA. Excess salts, proteins, phenol, or other contaminants in a DNA preparation can stress cells and reduce DNA uptake. Therefore, clean plasmid DNA can improve transformation efficiency and cell survival. Option B is incorrect because contaminants do not always increase efficiency; they often reduce it. Exam tip: link DNA purity in transformation with uptake and cell viability.

Related tags

TransformationPlasmid DnaCompetent CellsDna PurityTransformation EfficiencyCompetent HostPrinciples Of BiotechnologyBiotechnology

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