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Why is sterile technique important in transformation?

Answer and explanation

Correct answer: To prevent contamination

Sterile technique is used to keep the transformation experiment free from unwanted microorganisms and foreign DNA. If bacteria or fungi contaminate the culture or agar plate, they may grow as colonies, consume nutrients, or introduce DNA that is unrelated to the intended transformant. This can produce false-positive colonies and make colony counting, selection, and interpretation unreliable. Option C is correct because sterile handling protects the identity of the experimental sample and helps ensure that colonies arose from the intended host and transformation procedure. The other options describe no valid purpose of aseptic technique in molecular biology.

Related tags

Sterile TechniqueContaminationTransformationCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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