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Which option gives the correct role of antibiotic in cloning vector selection?

Answer and explanation

Correct answer: Helps select cells with marker

An antibiotic is used as a selective pressure in cloning experiments. The cloning vector carries an antibiotic-resistance marker, and cells that receive the vector can survive and grow on a medium containing the matching antibiotic. Cells that do not receive the vector usually lack this resistance and are prevented from growing under the selection condition.

Thus the antibiotic helps researchers select cells carrying the marker, which is option A. It does not join DNA strands; DNA ligase performs that function. It does not determine the insert sequence, and it does not create the origin of replication, or ori. Antibiotic selection identifies suitable transformed cells after the transformation step.

Related tags

Antibiotic SelectionMarkerTransformed Cells

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