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Which issue can affect insert stability in a cloning vector?

Answer and explanation

Correct answer: Insert being unstable in the host

Insert stability means that the foreign DNA remains intact and is maintained in the host without frequent deletion, rearrangement, or loss. An insert may be unstable when it is toxic to the host, contains repeated sequences, or places a heavy metabolic burden on the cell. Therefore, the vector–host combination and the copy number of the vector must be selected carefully. Option A is correct because instability of the insert in the host can reduce recovery of the intended clone and produce unreliable expression. Water colour, plate smell, and protein taste are unrelated observations and cannot explain genetic stability in a cloning experiment.

Related tags

Insert StabilityCloning VectorHost SelectionCloning VectorsPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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