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Which feature is most directly linked with insert DNA entry?

Answer and explanation

Correct answer: MCS or cloning site

The direct answer is A: MCS or cloning site. The MCS is a deliberately designed region of a cloning vector where foreign DNA is inserted. Step by step, a suitable restriction site in the MCS is used to open the vector, the insert is prepared with compatible ends, and ligase seals the insert into that position. Therefore MCS is directly linked with entry of insert DNA. A is correct. B, ori only, controls replication of the vector inside a host; it is not the normal insertion region. C, the host ribosome, translates mRNA into protein and is not a part of the vector’s DNA insertion design. D, antibiotic medium is used to select or grow certain transformed cells; it is not a vector feature and does not provide the insertion site. Do not confuse insertion with replication or selection. Memory cue: MCS receives the insert, ori copies the vector, and a marker helps select cells.

Related tags

McsInsert EntryCloning Site

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