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Which option correctly distinguishes selection and screening?

Answer and explanation

Correct answer: Selection chooses transformed cells and screening identifies recombinants

The governing concept is the difference between selection and screening during recombinant-DNA cloning. Selection applies a condition, commonly an antibiotic, so that cells carrying a vector survive or grow; cells without the selectable marker are removed from consideration. Screening is a second identification step used among the surviving cells to determine which ones also contain the desired insert. For example, disruption of lacZ may produce white colonies, helping identify likely recombinants. Therefore option A is correct: selection enriches for transformed cells, whereas screening distinguishes recombinants from non-recombinant transformants. The other options describe unrelated processes and do not explain vector uptake or insert identification.

Related tags

SelectionScreeningRecombinant DnaCloning VectorsPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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