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A vector has a marker but no ori. What problem will occur in host?

Answer and explanation

Correct answer: The vector will not replicate in the host cell

The ori (origin of replication) is the sequence from which replication of a vector begins. A marker gene may help in selecting transformed cells, but without an ori the vector cannot replicate and be maintained stably in the host cell. Therefore, option B is correct. A marker gene cannot perform the function of an ori. Exam tip: Remember the key vector components—ori for replication, a selectable marker for selection, and a cloning site for insertion of DNA.

Related tags

Cloning VectorsOrigin Of ReplicationOriSelectable MarkerVector ReplicationBiotechnologyPrinciples Of BiotechnologyClass 11 Mcq

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