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Which vector can be used for very large DNA inserts?

Answer and explanation

Correct answer: YAC

YAC stands for yeast artificial chromosome. It contains functional yeast chromosome elements, such as a centromere, telomere sequences, and an origin of replication, allowing it to behave like a small artificial chromosome in yeast. Because of this chromosome-like organization, YACs can accommodate very large DNA inserts, larger than those normally carried by ordinary plasmids. Option A is correct. pUC plasmids are typically small, high-copy cloning vectors and are not designed for very large fragments. Pepsin and amylase are digestive enzymes, not vectors, so they cannot replicate or maintain a DNA insert.

Related tags

YacVery Large InsertYeast VectorCloning VectorsPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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