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When is a promoter more necessary in a cloning vector?

Answer and explanation

Correct answer: When gene expression is needed

A promoter is a regulatory DNA sequence where RNA polymerase and associated factors begin transcription. It is especially necessary when the purpose of the vector is not merely to replicate an inserted DNA fragment but to express that gene and produce its RNA or protein in the host. The promoter must usually be recognized by the host’s transcription machinery and placed in a suitable orientation and position relative to the gene. Option A is correct. Water requirement, vector removal, and burning a host do not require transcriptional regulation and are unrelated to promoter function.

Related tags

PromoterExpression VectorGene ExpressionCloning VectorsPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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