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Which part is needed for independent replication inside the host in a cloning vector?

Answer and explanation

Correct answer: Ori (origin of replication)

Ori (origin of replication) is the specific DNA sequence from which replication of vector DNA begins inside the host cell. It is therefore essential for autonomous replication and maintenance of vector copies. A selectable marker only helps identify cells that have taken up the vector; it does not initiate replication. Exam tip: Remember that Ori is linked with replication, whereas a selectable marker is linked with selection of transformants.

Related tags

Cloning VectorsOrigin Of ReplicationOriVector ReplicationSelectable MarkerBiotechnologyPrinciples Of BiotechnologyClass 11 Mcq

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