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How do copies of desired DNA increase through a cloning vector?

Answer and explanation

Correct answer: The vector replicates in the host

The governing concept is amplification by replication inside a suitable host cell. A cloning vector, usually a plasmid, contains an origin of replication, or ori, that allows it to be copied by the host’s replication machinery. When the recombinant vector carrying the desired DNA insert replicates, the insert is copied along with the vector. Repeated cell growth and division can therefore produce many copies of the target sequence. Option A is correct because vector replication directly explains the increase. Burning destroys DNA, protein dissolution does not copy DNA, and freezing water has no role in molecular cloning.

Related tags

Desired Dna CopiesVector ReplicationHostCloning VectorsPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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