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Which option gives a wrong use of ori?

Answer and explanation

Correct answer: Selection of transformed cells

ori (origin of replication) is the DNA sequence from which replication of a vector begins. It can also influence the vector copy number and is necessary for autonomous replication in the host cell. Transformed cells are not selected by ori; selection is carried out using a selectable marker, such as an antibiotic-resistance gene. Exam tip: associate ori with replication and copy number, and selectable markers with selection.

Related tags

Cloning VectorsOrigin Of ReplicationOriSelectable MarkerVector ReplicationCopy NumberPrinciples Of BiotechnologyBiotechnology

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