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Which option is a wrong use of selectable marker in cloning vector?

Answer and explanation

Correct answer: Directly cutting a DNA fragment

A selectable marker helps identify or select cells that have taken up the cloning vector. For example, cells carrying a vector with an antibiotic-resistance gene can grow on a medium containing that antibiotic. Direct cutting of a DNA fragment is not the function of a selectable marker; it is done by a restriction endonuclease. Exam tip: associate selectable markers with selection and restriction enzymes with DNA cutting.

Related tags

Cloning VectorsSelectable MarkerAntibiotic ResistanceRestriction EnzymeTransformationPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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