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Why can a promoter be added to a cloning vector?

Answer and explanation

Correct answer: To enable gene expression

A promoter is a regulatory DNA sequence that provides a recognition and initiation region for transcription by RNA polymerase, often with the help of transcription factors. Placing a suitable promoter upstream of an inserted coding sequence can allow that gene to be transcribed in the chosen host, and in an expression vector this may lead to production of the corresponding RNA and protein. Therefore option A is correct. A promoter does not cut DNA; restriction enzymes perform cutting. It does not remove the host or freeze water. Promoter activity depends on its compatibility with the host and on regulatory conditions, so merely adding a promoter does not guarantee expression. Its intended function is to initiate or regulate transcription.

Related tags

PromoterGene ExpressionTranscriptionExpression VectorCloning VectorsPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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