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Which vector can be considered more suitable for very large DNA fragments?

Answer and explanation

Correct answer: YAC

The direct answer is A, YAC. A very large DNA fragment needs a carrier that can hold much more DNA than an ordinary small plasmid. YAC means yeast artificial chromosome. It contains chromosome-like features that allow large foreign DNA fragments to be maintained in yeast, so it is suitable for very large genomic inserts. Option A works because YACs were designed for large DNA cloning. Option B, a small pUC plasmid, is useful for ordinary smaller inserts but has a much lower carrying capacity, so it is not the best choice here. Option C, pepsin, is a protein-digesting enzyme, not a DNA vector. Option D, amylase, digests starch and is also not a vector. Exam cue: artificial chromosomes are chosen when the insert is too large for a normal plasmid.

Related tags

YacVery Large DnaCloning Vector

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