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Why is high copy number of pUC vector useful?

Answer and explanation

Correct answer: More plasmid DNA can be obtained

A plasmid with a high copy number can replicate many times inside a suitable host cell. As a result, each transformed cell may contain many copies of the pUC vector rather than only one or a few copies. This property is useful when the experiment requires isolation, analysis, sequencing, or other work with plasmid DNA.

If many cells carry the vector and each cell contains many copies, the total amount of recoverable plasmid DNA can increase substantially. Therefore option A, more plasmid DNA can be obtained, is correct. High copy number does not mean that less water is produced, that proteins always break, or that the host produces light. It specifically improves the possible plasmid DNA yield under suitable growth conditions.

Related tags

PucHigh Copy NumberDna Yield

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