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In pBR322 genes like ampR and tetR are examples of what?

Answer and explanation

Correct answer: Selectable markers

The direct answer is option A: ampR and tetR are selectable markers. In pBR322, ampR gives resistance to the antibiotic ampicillin and tetR gives resistance to tetracycline. When bacteria are treated to receive the plasmid, the antibiotic can be used to select cells that contain the vector: cells without the relevant resistance gene usually fail to grow under that condition. Thus these genes help identify or select transformed cells. Option A is correct. Option B is wrong because photosynthetic pigments, such as chlorophyll, capture light and are not antibiotic-resistance genes. Option C is wrong because digestive enzymes break food molecules and do not provide plasmid selection. Option D is wrong because water channels are membrane proteins, not the ampR or tetR genes of pBR322. A useful distinction is that a selectable marker gives survival or growth advantage under a chosen condition; it is not necessarily the inserted gene of interest. Exam cue: ampR means ampicillin resistance, and tetR means tetracycline resistance.

Related tags

Pbr322AmprTetrSelectable Markers

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