In blue-white screening, what does a blue colony generally show?Blue-white screening में blue colony सामान्यतः क्या दर्शाती है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: lacZ is active and the insert has not disrupted itlacZ is active and the insert has not disrupted it
A blue colony in blue-white screening generally indicates that the lacZ reporter remains intact and active. The active lacZ product acts on the indicator substrate and generates the blue colour, so the cloning site was usually not disrupted by a foreign DNA insert; this is interpreted as a likely non-recombinant colony. Hence option A is correct. A blue colour does not mean that DNA is absent, as stated in B. It is unrelated to chlorophyll in the host, ruling out C, and a vector remains DNA rather than becoming a protein, ruling out D. White colonies more commonly indicate disrupted lacZ and possible recombinants.Blue-white screening में blue colony सामान्यतः यह दिखाती है कि lacZ reporter gene intact और active है। Active lacZ product indicator substrate पर कार्य करके नीला रंग बनाता है। इसका अर्थ प्रायः यह होता है कि foreign DNA insert ने cloning site के भीतर lacZ को disrupt नहीं किया; इसलिए colony likely non-recombinant vector वाली मानी जाती है। अतः विकल्प A सही है। Blue रंग का अर्थ DNA का पूरी तरह absent होना नहीं है, इसलिए B गलत है। इसका host cell में chlorophyll होने से कोई संबंध नहीं, इसलिए C गलत है। Vector DNA ही रहता है, protein में नहीं बदलता, इसलिए D भी गलत है। इसके विपरीत, white colony अक्सर disrupted lacZ और संभावित recombinant को दर्शाती है।
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