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If a marker gene becomes inactive after insertion of foreign DNA into a cloning vector, what is this called?

Answer and explanation

Correct answer: Insertional inactivation

Insertional inactivation occurs when the inserted foreign DNA interrupts the coding sequence or regulatory region of a marker gene in a cloning vector. Because the gene is disrupted, its normal product is no longer made or its activity is lost. Researchers can use this change as a screening signal to identify vectors that contain the insert. Therefore option A is correct. Translation is the process of making a protein from mRNA, not the loss of marker activity. Evaporation is a physical change of liquid to vapour, and transpiration is water loss from plant surfaces; neither describes gene disruption caused by DNA insertion.

Related tags

Insertional InactivationMarker GeneScreeningCloning VectorsPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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