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Which part of a cloning vector can affect DNA copy number?

Answer and explanation

Correct answer: Origin of replication (Ori)

The origin of replication (Ori) is the sequence from which vector DNA replication begins. The nature and regulation of the Ori can affect the number of vector copies maintained in a cell. A selectable marker helps identify cells carrying the vector, but it does not directly control copy number. Exam tip: Associate Ori in a cloning vector with replication and copy-number control.

Related tags

Cloning VectorsOrigin Of ReplicationOriCopy NumberDna ReplicationBiotechnologyPrinciples Of BiotechnologyClass 11 Mcq

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