What is the importance of the lacZ gene in blue-white selection?Blue-white selection में lacZ gene का क्या महत्व है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: It helps distinguish recombinant and non-recombinant coloniesIt helps distinguish recombinant and non-recombinant colonies
In blue-white screening, a cloning vector commonly carries a lacZ reporter region containing a multiple-cloning site. If foreign DNA is inserted into that region, lacZ activity is disrupted and the recombinant colony usually appears white on the indicator medium. If the lacZ region remains intact, the enzyme activity produces a blue colony under the appropriate substrate conditions, indicating a likely non-recombinant vector. Therefore option A is correct. Option B describes restriction enzymes, while heating cells is a transformation treatment rather than a lacZ function. Protein digestion in D is unrelated to this reporter-based screening method.Blue-white screening में cloning vector के lacZ reporter region के भीतर अक्सर multiple-cloning site होती है। यदि foreign DNA इस स्थान पर insert हो जाए, तो lacZ gene की activity disrupt हो जाती है और उपयुक्त indicator medium पर recombinant colony सामान्यतः सफेद दिखाई देती है। यदि lacZ region intact रहे, तो enzyme activity के कारण colony नीली बनती है और वह प्रायः non-recombinant vector को दर्शाती है। इसलिए विकल्प A सही है। विकल्प B restriction enzyme का कार्य है। Host cells को गर्म करना transformation की एक प्रक्रिया हो सकती है, lacZ का कार्य नहीं। Protein digestion भी इस reporter-based screening से असंबंधित है।
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