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Which is a correct example of a cloning vector?

Answer and explanation

Correct answer: pBR322

The direct answer is option A: pBR322. A cloning vector is a DNA molecule used to carry a desired DNA fragment into a host cell and allow its maintenance or copying. A useful plasmid vector generally has an origin of replication, selectable marker genes, and suitable cloning sites. pBR322 is a well-known bacterial plasmid cloning vector containing an origin of replication and selectable markers such as antibiotic-resistance genes. Option A is correct because it is an actual plasmid vector used in recombinant-DNA work. Option B, amylase, is an enzyme that digests starch; it is a protein product, not a DNA carrier. Option C, insulin, is a hormone or protein, not a vector. Option D, chlorophyll, is a photosynthetic pigment, not a DNA molecule that carries an insert. A common mistake is to confuse any biotechnology-related substance with a vector. The word vector means a carrier, so in cloning it must be a suitable nucleic-acid molecule, commonly a plasmid, virus, BAC, or related construct. Memory cue: pBR322 is a classic plasmid carrier with replication and selection features.

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Pbr322Plasmid VectorExample

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