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Why is a buffer used in culture medium?

Answer and explanation

Correct answer: To keep pH relatively stable

The governing concept is pH buffering. A buffer contains a weak acid and its conjugate base, or a related chemical pair, that can absorb limited additions of acid or alkali and thereby resist sudden pH changes. Microbial metabolism often produces acidic or basic substances, so an uncontrolled culture may move away from the pH needed for growth, enzyme activity and product formation. Option D is correct because the buffer keeps pH relatively stable, though it does not hold it perfectly constant under unlimited disturbance. It is not primarily a killing agent, a sweetener or a material for converting product into metal. Larger pH corrections may still require controlled addition of acid or base.

Related tags

BiotechnologyBioprocess EngineeringBufferPh ControlPrinciples Of BiotechnologyClass 11 Mcq

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