15 results found for "lacZ" in all classes.
What is one use of lacZ reporter?
A. Blue-white screening
lacZ activity colony color screening में मदद कर सकती है। Insert disruption white colony दे सकता है। / lacZ activity can help in colony color screening. Insert disruption can give white colony.
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In which vector is lacZ-based blue-white screening commonly studied?
A. pUC vectors
pUC vectors lacZ आधारित blue-white screening से जुड़े होते हैं। यह recombinant screening का सरल example है। / pUC vectors are linked with lacZ-based blue-white screening. This is a simple example of recombinant screening.
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What can lacZ disruption indicate in blue-white screening?
A. रिकॉम्बिनेंट cloneRecombinant clone
Insert lacZ gene को disrupt कर सकता है। इससे white colony बनकर recombinant clone का संकेत मिल सकता है। / An insert can disrupt the lacZ gene. This can produce a white colony indicating a recombinant clone.
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What can happen when an insert enters the lacZ gene in blue-white screening?
A. lacZ inactive हो सकता हैlacZ can become inactive
Insert lacZ को disrupt कर सकता है। इससे white colony बन सकती है जो recombinant का संकेत हो सकती है। / An insert can disrupt lacZ. This may produce a white colony indicating a recombinant.
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lacZ disruption in a cloning vector is linked with which selection system?
A. नीले-सफेद चयनBlue-white selection
lacZ gene में insert आने से blue-white screening में colony color बदलता है। इससे recombinants पहचाने जाते हैं। / Insertion into lacZ changes colony color in blue-white screening. This helps identify recombinants.
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What is the importance of lacZ gene in blue-white selection?
A. Recombinant और non-recombinant colonies अलग करने में मददHelps distinguish recombinant and non-recombinant colonies
lacZ disruption से colony color बदल सकता है। इससे recombinant colonies पहचानने में मदद मिलती है। / Disruption of lacZ can change colony color. This helps identify recombinant colonies.
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lacZ reporter is linked with which screening?
A. नीला-सफेद screeningBlue-white screening
लैकजेड activity colony color बदल सकती है। Insert disruption white colony का संकेत दे सकता है। / lacZ activity can change colony color. Insert disruption can indicate a white colony.
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Why can a reporter gene be disrupted when an insert enters the MCS?
A. MCS reporter gene के अंदर हो सकता हैMCS can be inside the reporter gene
कई vectors में MCS lacZ region में होता है। Insert आने पर reporter activity disrupt हो सकती है। / In many vectors the MCS is in the lacZ region. An insert can disrupt reporter activity.
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In blue-white screening what can a blue colony generally indicate?
A. Non-recombinant vector
Blue colony अक्सर intact lacZ के कारण non-recombinant vector दिखाती है। Screening result को confirmation से verify करें। / A blue colony often indicates non-recombinant vector due to intact lacZ. Verify screening results by confirmation.
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In blue-white screening what can a white colony often indicate?
A. Insert disruption वाला recombinant cloneRecombinant clone with insert disruption
lacZ disruption white colony का संकेत दे सकता है। फिर भी final confirmation molecular method से करें। / lacZ disruption can indicate a white colony. Still final confirmation should be done by molecular methods.
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What is the main use of a reporter gene?
A. Expression या transformation signal दिखानाShowing expression or transformation signal
Reporter gene visible या measurable signal दे सकता है। GFP और lacZ common examples हैं। / A reporter gene can provide visible or measurable signal. GFP and lacZ are common examples.
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Which feature can help identify recombinant clones quickly?
A. Screening marker like lacZ
lacZ जैसा screening marker recombinants और non-recombinants में अंतर दिखा सकता है। इससे पहचान तेज होती है। / A screening marker like lacZ can distinguish recombinants and non-recombinants. This speeds up identification.
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In blue-white selection what does a blue colony generally indicate?
A. Non-recombinant vector
Blue colony lacZ active रहने का संकेत हो सकती है। इसका अर्थ सामान्यतः insert न होना है। / A blue colony can indicate active lacZ. It generally means the insert is absent.
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Which result can indicate a non-recombinant vector in blue-white screening?
A. Blue colony
Blue colony lacZ active होने का संकेत हो सकती है। सामान्यतः यह insert न होने वाले non-recombinant vector को दिखाती है। / A blue colony can indicate active lacZ. It generally shows a non-recombinant vector without insert.
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In blue-white screening what does a blue colony generally show?
A. lacZ active है और insert नहीं जुड़ाlacZ is active and insert is not inserted
Blue colony lacZ activity को दिखा सकती है। इसका अर्थ सामान्यतः non-recombinant vector हो सकता है। / A blue colony can show lacZ activity. It generally indicates a non-recombinant vector.
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