A. cytosine methylation in certain bacterial sequences
Step 1
Concept
Dcm methylation is linked with cytosine modification in bacterial DNA. Some restriction enzymes can be affected by it.
Step 2
Why this answer is correct
The correct answer is A. cytosine methylation in certain bacterial sequences. Dcm methylation is linked with cytosine modification in bacterial DNA. Some restriction enzymes can be affected by it.
Step 3
Exam Tip
Dcm methylation bacterial DNA में cytosine modification से जुड़ी है। कुछ restriction enzymes इससे प्रभावित हो सकते हैं।
A. suitable methylation-deficient host strain से/From a suitable methylation-deficient host strain
Step 1
Concept
A methylation-deficient host can make DNA suitable for some sensitive enzymes. Check enzyme requirements first.
Step 2
Why this answer is correct
The correct answer is A. suitable methylation-deficient host strain से / From a suitable methylation-deficient host strain. A methylation-deficient host can make DNA suitable for some sensitive enzymes. Check enzyme requirements first.
Step 3
Exam Tip
Methylation-deficient host कुछ sensitive enzymes के लिए DNA suitable बना सकता है। Enzyme requirement पहले check करें।
Dam methylation is linked with adenine modification in bacterial DNA. It can affect cutting by some enzymes.
Step 2
Why this answer is correct
The correct answer is A. adenine methylation in certain bacterial sites. Dam methylation is linked with adenine modification in bacterial DNA. It can affect cutting by some enzymes.
Step 3
Exam Tip
Dam methylation bacterial DNA में adenine modification से जुड़ी है। यह कुछ enzyme cutting को प्रभावित कर सकती है।
A. methylation-sensitive और insensitive enzymes compare करके/By comparing methylation-sensitive and insensitive enzymes
Step 1
Concept
If a methylation-sensitive enzyme does not cut methylation can be a cause. Alternative enzyme or host source can help.
Step 2
Why this answer is correct
The correct answer is A. methylation-sensitive और insensitive enzymes compare करके / By comparing methylation-sensitive and insensitive enzymes. If a methylation-sensitive enzyme does not cut methylation can be a cause. Alternative enzyme or host source can help.
Step 3
Exam Tip
अगर methylation-sensitive enzyme cut न करे तो methylation cause हो सकता है। Alternative enzyme या host source मदद कर सकता है।
A. DNA source और methylation status/DNA source and methylation status
Step 1
Concept
Methylated DNA can block enzyme cutting. Therefore a suitable host strain or alternative enzyme can be chosen.
Step 2
Why this answer is correct
The correct answer is A. DNA source और methylation status / DNA source and methylation status. Methylated DNA can block enzyme cutting. Therefore a suitable host strain or alternative enzyme can be chosen.
Step 3
Exam Tip
Methylated DNA enzyme cutting रोक सकता है। इसलिए suitable host strain या alternative enzyme चुना जा सकता है।
A. host DNA को own restriction enzyme से बचाना/Protecting host DNA from its own restriction enzyme
Step 1
Concept
When host DNA is methylated the restriction enzyme does not cut it. Foreign unmethylated DNA can be cut.
Step 2
Why this answer is correct
The correct answer is A. host DNA को own restriction enzyme से बचाना / Protecting host DNA from its own restriction enzyme. When host DNA is methylated the restriction enzyme does not cut it. Foreign unmethylated DNA can be cut.
Step 3
Exam Tip
Host DNA methylated होने पर restriction enzyme उसे नहीं काटता। Foreign unmethylated DNA काटा जा सकता है।
A. methylated site पर cutting बदल सकती है/Its cutting can change at methylated site
Step 1
Concept
Methylation-sensitive enzymes may cut methylated DNA poorly or not at all. This can change digestion pattern.
Step 2
Why this answer is correct
The correct answer is A. methylated site पर cutting बदल सकती है / Its cutting can change at methylated site. Methylation-sensitive enzymes may cut methylated DNA poorly or not at all. This can change digestion pattern.
Step 3
Exam Tip
Methylation-sensitive enzymes methylated DNA को कम या नहीं काट सकते। यह digestion pattern बदल सकता है।
A. methylation enzyme recognition रोक सकती है/Methylation can block enzyme recognition
Step 1
Concept
Some enzymes do not cut methylated recognition sites. Methylation sensitivity should be checked in enzyme choice.
Step 2
Why this answer is correct
The correct answer is A. methylation enzyme recognition रोक सकती है / Methylation can block enzyme recognition. Some enzymes do not cut methylated recognition sites. Methylation sensitivity should be checked in enzyme choice.
Step 3
Exam Tip
कुछ enzymes methylated recognition sites नहीं काटते। Methylation sensitivity enzyme choice में देखनी चाहिए।
A. enzyme recognition या cutting को प्रभावित कर सकती है/It can affect enzyme recognition or cutting
Step 1
Concept
Some restriction enzymes do not cut methylated sites. Therefore methylation status can change digestion results.
Step 2
Why this answer is correct
The correct answer is A. enzyme recognition या cutting को प्रभावित कर सकती है / It can affect enzyme recognition or cutting. Some restriction enzymes do not cut methylated sites. Therefore methylation status can change digestion results.
Step 3
Exam Tip
कुछ restriction enzymes methylated sites नहीं काटते। इसलिए methylation status digestion result बदल सकता है।
A. Host restriction enzymes DNA recognition को प्रभावित कर सकते हैं/Host restriction enzymes can be affected by DNA recognition
Step 1
Concept
Methylation can protect DNA from some restriction systems or change digestion pattern. It depends on the host system.
Step 2
Why this answer is correct
The correct answer is A. Host restriction enzymes DNA recognition को प्रभावित कर सकते हैं / Host restriction enzymes can be affected by DNA recognition. Methylation can protect DNA from some restriction systems or change digestion pattern. It depends on the host system.
Step 3
Exam Tip
Methylation कुछ restriction systems से DNA को बचा सकती है या digestion pattern बदल सकती है। यह host system पर निर्भर करता है।
Methylation can prevent some restriction enzymes from cutting. This may change expected bands.
Step 2
Why this answer is correct
The correct answer is A. कुछ sites cut न हों / Some sites may not cut. Methylation can prevent some restriction enzymes from cutting. This may change expected bands.
Step 3
Exam Tip
Methylation कुछ restriction enzymes को cut करने से रोक सकती है। इससे expected bands बदल सकते हैं।
A. प्रतिबंध एंजाइम उसे काट सकते हैं/Restriction enzymes may cut it
Step 1
Concept
Without methylation foreign DNA is not protected like self DNA. Therefore restriction enzymes may cut it.
Step 2
Why this answer is correct
The correct answer is A. प्रतिबंध एंजाइम उसे काट सकते हैं / Restriction enzymes may cut it. Without methylation foreign DNA is not protected like self DNA. Therefore restriction enzymes may cut it.
Step 3
Exam Tip
मेथिलेशन न होने पर विदेशी डीएनए स्वयं DNA जैसा सुरक्षित नहीं रहता। इसलिए restriction enzymes उसे काट सकते हैं।
A. methylation recognition या cutting रोक सकती है/Methylation can block recognition or cutting
Step 1
Concept
Some enzymes do not cut methylated recognition sites. Therefore methylation sensitivity must be checked.
Step 2
Why this answer is correct
The correct answer is A. methylation recognition या cutting रोक सकती है / Methylation can block recognition or cutting. Some enzymes do not cut methylated recognition sites. Therefore methylation sensitivity must be checked.
Step 3
Exam Tip
कुछ enzymes methylated recognition sites नहीं काटते। इसलिए methylation sensitivity देखना जरूरी है।
Host DNA methylation can protect its own recognition sites. Foreign unmethylated DNA can be cut.
Step 2
Why this answer is correct
The correct answer is A. methylation द्वारा / By methylation. Host DNA methylation can protect its own recognition sites. Foreign unmethylated DNA can be cut.
Step 3
Exam Tip
Host DNA methylation own recognition sites को protect कर सकती है। Foreign unmethylated DNA cut हो सकता है।
A. buffer temperature methylation sensitivity और heat inactivation जानने के लिए/To know buffer temperature methylation sensitivity and heat inactivation
Step 1
Concept
Each enzyme can have different working conditions. Official data helps correct reaction setup.
Step 2
Why this answer is correct
The correct answer is A. buffer temperature methylation sensitivity और heat inactivation जानने के लिए / To know buffer temperature methylation sensitivity and heat inactivation. Each enzyme can have different working conditions. Official data helps correct reaction setup.
Step 3
Exam Tip
हर enzyme की working condition अलग हो सकती है। Official data सही reaction setup में मदद करता है।
A. test DNA में site absent या methylated हो सकती है/Test DNA may lack site or be methylated
Step 1
Concept
Positive control shows enzyme activity. Test DNA may have recognition site or methylation issue.
Step 2
Why this answer is correct
The correct answer is A. test DNA में site absent या methylated हो सकती है / Test DNA may lack site or be methylated. Positive control shows enzyme activity. Test DNA may have recognition site or methylation issue.
Step 3
Exam Tip
Positive control enzyme activity दिखाता है। Test DNA में recognition site या methylation issue हो सकता है।
C. host DNA को methylate करना/Methylating host DNA
Step 1
Concept
Modification is usually linked with methylation of host DNA. This can protect host DNA from cutting.
Step 2
Why this answer is correct
The correct answer is C. host DNA को methylate करना / Methylating host DNA. Modification is usually linked with methylation of host DNA. This can protect host DNA from cutting.
Step 3
Exam Tip
Modification usually host DNA methylation से जुड़ी है। इससे host DNA cutting से बच सकता है।
Bacteria can methylate their recognition sites. This protects their own DNA from restriction enzymes.
Step 2
Why this answer is correct
The correct answer is B. methylation द्वारा / By methylation. Bacteria can methylate their recognition sites. This protects their own DNA from restriction enzymes.
Step 3
Exam Tip
Bacteria अपने recognition sites को methylate कर सकते हैं। इससे own DNA restriction enzyme से सुरक्षित रहता है।
A. अपना DNA restriction enzyme से cut हो सकता है/Its own DNA may be cut by restriction enzyme
Step 1
Concept
Methylation protects self DNA from restriction enzymes. Without it self DNA can become vulnerable.
Step 2
Why this answer is correct
The correct answer is A. अपना DNA restriction enzyme से cut हो सकता है / Its own DNA may be cut by restriction enzyme. Methylation protects self DNA from restriction enzymes. Without it self DNA can become vulnerable.
Step 3
Exam Tip
Methylation self DNA को restriction enzyme से बचाती है। इसके बिना own DNA भी vulnerable हो सकता है।
A. foreign DNA cut कर सकता है/It can cut foreign DNA
Step 1
Concept
If foreign DNA is not methylated the restriction enzyme can recognize and cut it. This is part of bacterial defense.
Step 2
Why this answer is correct
The correct answer is A. foreign DNA cut कर सकता है / It can cut foreign DNA. If foreign DNA is not methylated the restriction enzyme can recognize and cut it. This is part of bacterial defense.
Step 3
Exam Tip
Foreign DNA methylated न हो तो restriction enzyme उसे पहचानकर cut कर सकता है। यह bacterial defense का हिस्सा है।
A. methylase और methylation/Methylase and methylation
Step 1
Concept
Methylase can methylate bacterial DNA. This prevents restriction enzymes from cutting self DNA.
Step 2
Why this answer is correct
The correct answer is A. methylase और methylation / Methylase and methylation. Methylase can methylate bacterial DNA. This prevents restriction enzymes from cutting self DNA.
Step 3
Exam Tip
Methylase bacterial DNA को methylate कर सकता है। इससे restriction enzyme अपना DNA नहीं काटता।
A. cutting रुक या घट सकती है/Cutting may stop or decrease
Step 1
Concept
Methylation can block cutting by many restriction enzymes. This helps bacteria protect their own DNA.
Step 2
Why this answer is correct
The correct answer is A. cutting रुक या घट सकती है / Cutting may stop or decrease. Methylation can block cutting by many restriction enzymes. This helps bacteria protect their own DNA.
Step 3
Exam Tip
Methylation कई restriction enzymes की cutting रोक सकती है। इसी से bacteria अपना DNA बचाते हैं।
A. अपना DNA कटने से बचता है/Its own DNA is protected from cutting
Step 1
Concept
Methylation can protect self DNA from being cut. This is part of the restriction-modification system.
Step 2
Why this answer is correct
The correct answer is A. अपना DNA कटने से बचता है / Its own DNA is protected from cutting. Methylation can protect self DNA from being cut. This is part of the restriction-modification system.
Step 3
Exam Tip
मेथिलेशन self DNA को पहचानकर कटने से बचा सकता है। यह restriction-modification system का हिस्सा है।
A. कटिंग रुक या घट सकती है/Cutting may be blocked or reduced
Step 1
Concept
Methylation can block cutting by many restriction enzymes. Bacteria protect their own DNA in this way.
Step 2
Why this answer is correct
The correct answer is A. कटिंग रुक या घट सकती है / Cutting may be blocked or reduced. Methylation can block cutting by many restriction enzymes. Bacteria protect their own DNA in this way.
Step 3
Exam Tip
मेथिलेशन कई प्रतिबंध एंजाइमों की कटिंग को रोक सकता है। जीवाणु अपना DNA इसी प्रकार बचाते हैं।
A. अपने डीएनए की मेथिलेशन से रक्षा करना/Protecting self DNA by methylation
Step 1
Concept
The modification part methylates self DNA. This prevents the restriction enzyme from cutting self DNA.
Step 2
Why this answer is correct
The correct answer is A. अपने डीएनए की मेथिलेशन से रक्षा करना / Protecting self DNA by methylation. The modification part methylates self DNA. This prevents the restriction enzyme from cutting self DNA.
Step 3
Exam Tip
संशोधन भाग अपना डीएनए मेथिलेट करता है। इससे प्रतिबंध एंजाइम अपना डीएनए नहीं काटता।
Bacteria protect their own DNA by methylation. Foreign DNA can be cut if it is not methylated.
Step 2
Why this answer is correct
The correct answer is B. मेथिलेशन द्वारा / By methylation. Bacteria protect their own DNA by methylation. Foreign DNA can be cut if it is not methylated.
Step 3
Exam Tip
जीवाणु अपने डीएनए को मेथिलेशन से सुरक्षित रखते हैं। विदेशी डीएनए मेथिलेटेड न होने पर कट सकता है।
