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Biotechnology Restriction Enzymes MCQ Questions for Class 11 Science

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Restriction Enzymes Practice Questions

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Question 271/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 16

अगर insert और vector के sticky ends complementary हैं तो पहला weak joining किससे होता है?

If sticky ends of insert and vector are complementary what first weak joining occurs by?

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A. base pairing

Explanation

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Complementary sticky ends hydrogen bonding द्वारा base pairing कर सकते हैं। Permanent joining के लिए DNA ligase चाहिए। / Complementary sticky ends can base pair through hydrogen bonding. DNA ligase is needed for permanent joining.

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Question 272/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 16

restriction enzyme किस bond को DNA backbone में काटता है?

Which bond does a restriction enzyme cut in the DNA backbone?

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A. phosphodiester bond

Explanation

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Restriction enzymes DNA के sugar-phosphate backbone में phosphodiester bonds काटते हैं। यह DNA structure से जुड़ा fact है। / Restriction enzymes cut phosphodiester bonds in the sugar-phosphate backbone of DNA. This fact is linked with DNA structure.

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Question 273/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 16

किस option में restriction enzyme की protein nature का सही implication है?

Which option gives the correct implication of protein nature of restriction enzymes?

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A. heat से activity घट सकती हैHeat can reduce activity

Explanation

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Restriction enzymes protein enzymes हैं। अधिक heat या गलत conditions activity घटा सकती हैं। / Restriction enzymes are protein enzymes. High heat or wrong conditions can reduce activity.

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Question 274/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 16

किस temperature पर कई common bacterial restriction enzymes work करते हैं?

At what temperature do many common bacterial restriction enzymes work?

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A. लगभग 37 डिग्री सेल्सियसAbout 37 degrees Celsius

Explanation

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कई bacterial enzymes 37 डिग्री सेल्सियस के आसपास active रहते हैं। फिर भी exact optimum enzyme-specific हो सकता है। / Many bacterial enzymes are active around 37 degrees Celsius. However the exact optimum can be enzyme-specific.

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Question 275/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 16

restriction digestion में incubation का उद्देश्य क्या है?

What is the purpose of incubation in restriction digestion?

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A. enzyme को DNA cut करने का समय देनाGiving enzyme time to cut DNA

Explanation

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Incubation में enzyme उचित condition में DNA पर action करता है। Time और temperature practical factors हैं। / During incubation the enzyme acts on DNA under suitable conditions. Time and temperature are practical factors.

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Question 276/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 16

किस कारण एक छोटा चार-base recognition sequence genome में अधिक बार मिल सकता है?

Why can a short four-base recognition sequence occur more often in a genome?

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Correct Answer

A. क्योंकि छोटा sequence statistically अधिक frequent हो सकता हैBecause a shorter sequence can be statistically more frequent

Explanation

Simple Explanation

Short recognition sequences longer sequences की तुलना में अधिक बार मिल सकते हैं। इससे अधिक cut sites संभव हैं। / Short recognition sequences can occur more often than longer sequences. This can lead to more cut sites.

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Question 277/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 16

छह-base recognition sequence की तुलना में आठ-base sequence सामान्यतः क्या करेगा?

Compared with a six-base recognition sequence what will an eight-base sequence generally do?

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Correct Answer

A. कम frequent sites देगाGive less frequent sites

Explanation

Simple Explanation

Longer recognition sequence कम बार मिलने की संभावना रखता है। इससे fragments की संख्या प्रभावित हो सकती है। / A longer recognition sequence is less likely to occur frequently. This can affect the number of fragments.

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Question 278/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 16

अगर restriction enzyme inactive हो तो expected result क्या होगा?

If a restriction enzyme is inactive what is the expected result?

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A. DNA properly cut नहीं होगाDNA will not be cut properly

Explanation

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Inactive enzyme recognition site होने पर भी cut नहीं कर सकता। Activity restriction digestion की basic requirement है। / An inactive enzyme cannot cut even if the recognition site is present. Activity is a basic requirement of restriction digestion.

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Question 279/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 16

gel में uncut plasmid band दिखने का एक कारण क्या हो सकता है?

What can be one reason for seeing an uncut plasmid band in gel?

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A. incomplete digestion

Explanation

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Uncut band दिखे तो कुछ plasmid molecules cut नहीं हुए हो सकते हैं। यह incomplete digestion का संकेत हो सकता है। / If an uncut band appears some plasmid molecules may not have been cut. This can indicate incomplete digestion.

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Question 280/345 Easy Biotechnology Principles of Biotechnology Restriction Enzymes Class 11 Level 16

किस practical में restriction enzyme और gel electrophoresis साथ दिखेंगे?

In which practical will restriction enzyme and gel electrophoresis appear together?

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Correct Answer

A. DNA fragment analysis

Explanation

Simple Explanation

पहले restriction enzyme DNA cut करता है और फिर gel electrophoresis fragments अलग करता है। दोनों DNA analysis में जुड़े हैं। / First the restriction enzyme cuts DNA and then gel electrophoresis separates fragments. Both are linked in DNA analysis.

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