Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.
Difficulty select karke sirf is topic ke focused MCQs practice karein. Score, timer aur explanations student-friendly flow me milenge.
Showing 211-220 of 445 questions.
A. Isolated colonies पाने के लिएTo obtain isolated colonies
Streaking dense culture से अलग colonies प्राप्त करने में मदद करता है। Pure clone selection में यह useful है। / Streaking helps obtain separate colonies from a dense culture. It is useful in pure clone selection.
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Which analysis is common after miniprep?
A. Restriction digestion या sequencingRestriction digestion or sequencing
Miniprep plasmid DNA देता है। फिर restriction digestion या sequencing से clone verify किया जाता है। / Miniprep gives plasmid DNA. Then the clone is verified by restriction digestion or sequencing.
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What does getting expected bands in a restriction digest indicate?
A. Correct clone हो सकता हैIt may be the correct clone
Expected bands vector map और insert size से match कर सकते हैं। यह correct clone का संकेत है। / Expected bands can match the vector map and insert size. This indicates a correct clone.
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What can a wrong band pattern indicate?
A. Wrong clone या rearrangementWrong clone or rearrangement
Wrong pattern insert absence wrong orientation या rearrangement का संकेत हो सकता है। Further confirmation करें। / A wrong pattern can indicate insert absence wrong orientation or rearrangement. Do further confirmation.
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Why does sequencing give final confirmation?
A. Insert की exact sequence बता सकती हैIt can tell the exact insert sequence
Sequencing insert identity और mutation status दिखा सकती है। इसलिए यह reliable confirmation है। / Sequencing can show insert identity and mutation status. Therefore it is reliable confirmation.
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Why is a mutation-free insert important?
A. Desired gene function सही रखने के लिएTo keep desired gene function correct
Mutation gene function या protein product बदल सकती है। इसलिए sequencing confirmation उपयोगी है। / A mutation can change gene function or protein product. Therefore sequencing confirmation is useful.
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How can spontaneous mutation affect cloning results?
A. Insert या vector sequence बदल सकती हैIt can change insert or vector sequence
Mutation clone की sequence बदल सकती है। Final construct को verify करना जरूरी है। / A mutation can change the clone sequence. The final construct must be verified.
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Why is unwanted recombination a problem in cloning?
A. Insert rearrangement हो सकता हैInsert rearrangement can occur
Unwanted recombination cloned DNA को बदल सकती है। Recombination-deficient host stability में मदद करता है। / Unwanted recombination can change cloned DNA. A recombination-deficient host helps stability.
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What risk can be higher in an insert with repeated DNA sequences?
A. Recombination से rearrangementRearrangement by recombination
Repeated sequences recombination-prone हो सकती हैं। इसलिए suitable host strain जरूरी है। / Repeated sequences can be recombination-prone. Therefore a suitable host strain is important.
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Which strategy can help maintain a toxic insert?
A. Low-copy vector या controlled expressionLow-copy vector or controlled expression
Toxic insert host growth घटा सकता है। Low-copy या controlled expression burden कम कर सकते हैं। / A toxic insert can reduce host growth. Low-copy or controlled expression can reduce burden.
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