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Biotechnology Cloning Vectors MCQ Questions for Class 11 Science

Practice focused topic-wise MCQs with answers and explanations for quick revision and exam preparation.

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01 Practice Topic Genetic Engineering 200 MCQs 02 Practice Topic Bioprocess Engineering 300 MCQs 03 Practice Topic Restriction Enzymes 345 MCQs 04 Practice Topic Ligase Enzymes 290 MCQs 05 Practice Topic Competent Host 445 MCQs

Cloning Vectors Practice Questions

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Question 271/295 Easy Biotechnology Principles of Biotechnology Cloning Vectors Class 11 Level 29

कौन सा vector feature repeated subculture में vector loss घटा सकता है?

Which vector feature can reduce vector loss during repeated subculture?

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Correct Answer

A. Selectable marker with selection

Explanation

Simple Explanation

Selection marker और selection condition vector-containing cells को बनाए रखने में मदद करते हैं। इससे vector loss घट सकता है। / A selection marker and selection condition help maintain vector-containing cells. This can reduce vector loss.

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Question 272/295 Easy Biotechnology Principles of Biotechnology Cloning Vectors Class 11 Level 29

किस situation में host cell recombinant vector खो सकती है?

In which situation can a host cell lose recombinant vector?

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A. Selection pressure न होSelection pressure is absent

Explanation

Simple Explanation

Selection pressure न हो तो vector रखने वाली cells को लाभ नहीं मिलता। इससे vector loss हो सकता है। / Without selection pressure cells carrying the vector get no advantage. This can lead to vector loss.

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Question 273/295 Easy Biotechnology Principles of Biotechnology Cloning Vectors Class 11 Level 29

किस option में vector contamination की practical समस्या दिखती है?

Which option shows a practical problem of vector contamination?

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Correct Answer

A. गलत plasmid colony मिलनाGetting wrong plasmid colony

Explanation

Simple Explanation

गलत plasmid contamination से गलत clone मिल सकता है। इसलिए screening और sterile technique जरूरी हैं। / Wrong plasmid contamination can give a wrong clone. Therefore screening and sterile technique are important.

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Question 274/295 Easy Biotechnology Principles of Biotechnology Cloning Vectors Class 11 Level 29

किस test से recombinant vector का final भरोसेमंद confirmation मिल सकता है?

Which test can give reliable final confirmation of a recombinant vector?

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Correct Answer

A. DNA sequencing

Explanation

Simple Explanation

DNA sequencing insert identity और orientation confirm कर सकती है। इसलिए यह final confirmation के लिए मजबूत method है। / DNA sequencing can confirm insert identity and orientation. Therefore it is a strong method for final confirmation.

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Question 275/295 Easy Biotechnology Principles of Biotechnology Cloning Vectors Class 11 Level 29

Restriction digestion confirmation में wrong band pattern क्या बता सकता है?

What can a wrong band pattern in restriction digestion confirmation indicate?

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Correct Answer

A. गलत insert या गलत cloneWrong insert or wrong clone

Explanation

Simple Explanation

Wrong band pattern clone problem का संकेत हो सकता है। इसे insert size और vector map से compare करें। / A wrong band pattern can indicate a clone problem. Compare it with insert size and vector map.

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Question 276/295 Easy Biotechnology Principles of Biotechnology Cloning Vectors Class 11 Level 29

Vector map किस जानकारी में मदद करता है?

What information does a vector map help with?

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Correct Answer

A. Ori marker MCS और restriction sites की positionPositions of ori marker MCS and restriction sites

Explanation

Simple Explanation

Vector map important features की position दिखाता है। इससे cloning planning आसान होती है। / A vector map shows positions of important features. This makes cloning planning easier.

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Question 277/295 Easy Biotechnology Principles of Biotechnology Cloning Vectors Class 11 Level 29

किसके आधार पर restriction enzyme choose किया जा सकता है?

On what basis can a restriction enzyme be chosen?

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Correct Answer

A. Vector MCS और insert sequenceVector MCS and insert sequence

Explanation

Simple Explanation

Restriction enzyme choice MCS sites और insert में unwanted cut sites पर निर्भर करती है। यह cloning planning का हिस्सा है। / Restriction enzyme choice depends on MCS sites and unwanted cut sites within insert. This is part of cloning planning.

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Question 278/295 Easy Biotechnology Principles of Biotechnology Cloning Vectors Class 11 Level 29

यदि चुना गया enzyme insert के अंदर भी cut करता है तो क्या समस्या होगी?

If the chosen enzyme also cuts inside the insert what problem will occur?

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A. Insert टूट सकता हैInsert may break

Explanation

Simple Explanation

Insert के अंदर cut होने से desired gene fragment टूट सकता है। इसलिए enzyme selection से पहले insert sequence देखना जरूरी है। / A cut inside the insert can break the desired gene fragment. Therefore insert sequence should be checked before enzyme selection.

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Question 279/295 Easy Biotechnology Principles of Biotechnology Cloning Vectors Class 11 Level 29

किस cloning design में insert को दो अलग enzymes से काटना उपयोगी है?

In which cloning design is cutting insert with two different enzymes useful?

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Correct Answer

A. Directional cloning

Explanation

Simple Explanation

दो अलग enzymes insert के ends अलग बनाते हैं। इससे insert orientation control होता है। / Two different enzymes create different ends on the insert. This controls insert orientation.

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Question 280/295 Easy Biotechnology Principles of Biotechnology Cloning Vectors Class 11 Level 29

कौन सा vector part cloning planning में सबसे पहले map पर देखा जाता है?

Which vector part is often checked first on a map for cloning planning?

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Correct Answer

A. MCS restriction sites

Explanation

Simple Explanation

MCS बताता है कि कौन से restriction sites insert cloning के लिए उपलब्ध हैं। यह cloning strategy तय करने में मदद करता है। / MCS shows which restriction sites are available for insert cloning. It helps decide the cloning strategy.

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