अगर vector dephosphorylated है तो insert ligation के लिए क्या महत्वपूर्ण हो सकता है?
If vector is dephosphorylated what may be important for insert ligation?
Explanation opens after your attempt
Correct Answer
A. insert के पास सही phosphate ends होंInsert has proper phosphate ends
Concept
Dephosphorylated vector reduces self-ligation but proper insert ends help ligation. Chemical ends must be considered.
Why this answer is correct
The correct answer is A. insert के पास सही phosphate ends हों / Insert has proper phosphate ends. Dephosphorylated vector reduces self-ligation but proper insert ends help ligation. Chemical ends must be considered.
Exam Tip
Dephosphorylated vector self-ligation घटाता है लेकिन proper insert ends ligation में मदद करते हैं। Chemical ends का ध्यान जरूरी है।
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